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Catalog No: OCOA16390
Size:20UG
Price: $275.00
SKU
OCOA16390
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FXR1 Protein Lysate (OCOA16390)

Datasheets/ManualsPrintable datasheet for FXR1 Protein Lysate (OCOA16390)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionFXR1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolFXR1
Gene Full NameFMR1 autosomal homolog 1
Alias Symbolsfragile X mental retardation syndrome-related protein 1;fragile X mental retardation, autosomal homolog 1;FXR1P;MYOPMIL;MYORIBF.
NCBI Gene Id8087
Protein NameNM_001013439, NP_001013457, P51114
Description of TargetFragile X mental retardation syndrome-related protein 1, fragile X mental retardation syndrome-related protein 1 isoform c [Homo sapiens], Homo sapiens FMR1 autosomal homolog 1 (FXR1), transcript variant 3, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P51114
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001013457
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001013439
Molecular Weight59.7 kDa
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