FUT8 ELISA Kit (Mouse) (OKCD00959)

Catalog No: OKCD00959 (Formerly GWB-KBBPL6)

Size:96WELLS

Mouse Fut8 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	91-101%	80-101%	92-101%	98-105%
  EDTA plasma(n=5)	90-98%	80-90%	99-105%	85-105%
  heparin plasma(n=5)	89-96%	89-96%	79-95%	99-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosyltransferase 8 (FUT8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fucosyltransferase 8 (FUT8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosyltransferase 8 (FUT8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fucosyltransferase 8 (FUT8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	82-91	85
  EDTA plasma(n=5)	83-104	95
  heparin plasma(n=5)	90-98	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fucosyltransferase 8 (FUT8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fucosyltransferase 8 (FUT8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Fut8 Microplate: 96 Wells (12 x 8 Well Strips)
  • Fut8 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Fut8 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.127 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fucosyltransferase 8 (FUT8). No significant cross-reactivity or interference between Fucosyltransferase 8 (FUT8) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Fut8
• Gene Full Name: fucosyltransferase 8
• Alias Symbols: alpha-(1,6)-fucosyltransferase;alpha1-6FucT;Fucosyltransferase 8;GDP-fucose--glycoprotein fucosyltransferase;GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase;glycoprotein 6-alpha-L-fucosyltransferase.
• NCBI Gene Id: 53618
• Protein Name: Alpha-(1,6)-fucosyltransferase
• Description of Target: Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
• Uniprot ID: Q9WTS2
• Protein Accession #: NP_001239543
• Nucleotide Accession #: NM_001252614.1
• Protein Size (# AA): 575