FUT2 ELISA Kit (Human) (OKCD08753)

Catalog No: OKCD08753

Size:96WELLS

FUT2 ELISA Kit (Human) (OKCD08753) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosyltransferase 2 (FUT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fucosyltransferase 2 (FUT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosyltransferase 2 (FUT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fucosyltransferase 2 (FUT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fucosyltransferase 2 (FUT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fucosyltransferase 2 (FUT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FUT2 Microplate: 96 Wells (12 x 8 Well strips)
  • FUT2 Lyophilized Standard: 2
  • 100X FUT2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.122ng/mL

Target Info

• Gene Symbol: FUT2
• Gene Full Name: fucosyltransferase 2
• Alias Symbols: alpha (1,2) fucosyltransferase;alpha(1,2)FT 2;alpha(1,2)FT2;B12QTL1;Fucosyltransferase 2;fucosyltransferase 2 (secretor status included);galactoside 2-alpha-L-fucosyltransferase 2;galactoside 2-L-fucosyltransferase;galactoside alpha-(1,2)-fucosyltransferase 2;GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2;SE;Se2;SEC2;secretor blood group alpha-2-fucosyltransferase;secretor factor;sej;truncated fucosyltransferase 2;type 1 galactoside alpha-(1,2)-fucosyltransferase FUT2;type 2 galactoside alpha-(1,2)-fucosyltransferase FUT2.
• NCBI Gene Id: 2524
• Protein Name: Galactoside 2-alpha-L-fucosyltransferase 2
• Description of Target: Mediates the transfer of fucose to the terminal galactose on glycan chains of cell surface glycoproteins and glycolipids (PubMed:7876235). The resulting epitope plays a role in cell-cell interaction including host-microbe interaction (PubMed:12692541, PubMed:8018146). Mediates interaction with intestinal microbiota influencing its composition (PubMed:21625510, PubMed:24733310, PubMed:22068912). Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble ABO blood group antigen synthesis pathway (PubMed:7876235).
• Uniprot ID: Q10981
• Protein Accession #: NP_000502.4
• Nucleotide Accession #: NM_000511.5