FUS ELISA Kit (Mouse) (OKCD00319)

Catalog No: OKCD00319 (Formerly GWB-KBBBF1)

Size:96WELLS

Mouse Fus ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fusion (FUS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fusion (FUS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fusion (FUS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fusion (FUS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fusion (FUS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fusion (FUS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Fus Microplate: 96 Wells (12 x 8 Well Strips)
  • Fus Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Fus Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fusion (FUS). No significant cross-reactivity or interference between Fusion (FUS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Fus
• Gene Full Name: fused in sarcoma
• Alias Symbols: D430004D17Rik;D930039C12Rik;Fus1;fusion, derived from t(12;16) malignant liposarcoma;hnRNP P2;pi;pigpen protein;protein pigpen;RNA-binding protein FUS;T;Tls;translocated in liposarcoma.
• NCBI Gene Id: 233908
• Protein Name: RNA-binding protein FUS
• Description of Target: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity (By similarity).
• Uniprot ID: P56959
• Protein Accession #: NP_631888
• Nucleotide Accession #: NM_139149.2
• Protein Size (# AA): 518