FUR ELISA kit (Rat) (OKCD04268)

Catalog No: OKCD04268

Size:96WELLS

Rat FUR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Furin (FUR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Furin (FUR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Furin (FUR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Furin (FUR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	80-103	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Furin (FUR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Furin (FUR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Fur Microplate: 96 Wells (12 x 8 Well strips)
  • Fur Lyophilized Standard: 2
  • 100X Fur HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: 0.053 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for FUR ELISA kit (Rat) on CiteAb

Target Info

• Gene Symbol: Furin
• Gene Full Name: furin (paired basic amino acid cleaving enzyme)
• Alias Symbols: dibasic-processing enzyme;furin;Pace;Paired basic amino acid cleaving enzyme (furin);paired basic amino acid residue-cleaving enzyme;Pcsk3;prohormone convertase 3;Proprotein convertase subtilisin/kexin type 3 (paired basic amino acid cleaving enzyme, furin, membrane associated receptor protein);proprotein convertase subtilisin/kexin type3.
• NCBI Gene Id: 54281
• Protein Name: Furin
• Description of Target: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
• Uniprot ID: P23377
• Protein Accession #: NP_062204.1
• Nucleotide Accession #: NM_019331.1