FTO Recombinant Antibody [8M13] (ATM00001)

Catalog No: ATM00001

Size:100ug

20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjugated donkey anti-rabbit IgG antibody. Images were captured after development of membrane following exposure to AvivaBlot™ Femto ECL Reagent (OKCF00002).

Immunogen: Recombinant protein representing full length structure prediction (aa 328-505) of human FTO (green) via AlphaFold. (https://alphafold.ebi.ac.uk/).

Immunofluorescence staining of HeLa cells using ATM00001 at a dilution of 1:100

The 1.6mg/mL of antibody were placed on the HC30M chip in duplicates and amino-coupled using EDC/Sulfo-NHS chemistry. The chip was blocked by 1M Ethanolamine, pH 8.5 and washed with 1xHBSTE Buffer + 0.5mg/mL BSA (bovine serum albumin). Antigen was applied in 5 different concentrations (250nM, 125nM, 62.5nM, 31,25nM and 15.6nM) and fitted globally for association (900s = 15 min) and dissociation phases (1800s = 30min). The fitted curves are shown in red.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: PBS w/ 0.09% sodium azide
• Clonality: Recombinant Monoclonal
• Clone: 8M13
• Isotype: IgG
• Host: Rabbit
• Application: IF, WB
• Additional Information: This product was developed using Active Motif’s AbFlex® antibody technology.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: Recombinant Human FTO aa328-505
• Purification: Protein A
• Concentration: 1 mg/mL

Target Info

• Gene Symbol: FTO
• Gene Full Name: alpha-ketoglutarate-dependent dioxygenase
• Alias Symbols: FTO
• NCBI Gene Id: 79068
• Protein Name: Alpha-ketoglutarate-dependent dioxygenase FTO
• Description of Target: RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis (PubMed:22002720, PubMed:26458103, PubMed:28002401, PubMed:30197295, PubMed:26457839, PubMed:25452335). Specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103, PubMed:30197295, PubMed:26457839, PubMed:25452335), among other targets.
• Uniprot ID: Q9C0B1-1
• Protein Size (# AA): 505
• Molecular Weight: 58.3 kDa