FTO Polyclonal Antibody (Human) (ATP00003_T100)

Catalog No: ATP00003_T100

Size:100 uL

20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjugated donkey anti-rabbit IgG antibody. Images were captured after development of membrane following exposure to AvivaBlot™ Femto ECL Reagent (OKCF00002).

Immunogen: Recombinant protein representing aa 328-505 of human FTO (green) depicted within in the full length structure prediction (blue) via AlphaFold. (https://alphafold.ebi.ac.uk/).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: Recombinant Human FTO aa 328-505
• Purification: Protein A purified
• Concentration: 1 mg/mL

Target Info

• Gene Symbol: FTO
• Gene Full Name: Fat mass and obesity associated
• Alias Symbols: GDFD, ALKBH9, BMIQ14
• NCBI Gene Id: 79068
• Protein Name: Alpha-ketoglutarate-dependent dioxygenase FTO
• Description of Target: This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]
• Uniprot ID: Q9C0B1
• Protein Accession #: NP_001073901
• Nucleotide Accession #: NM_001080432
• Protein Size (# AA): 505
• Molecular Weight: 58 kDa