FTO ELISA Kit (Rat) (OKEH03710)

Catalog No: OKEH03710

Size:96T

Rat FTO ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology FTO ELISA Kit (Rat) (OKEH03710) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fto has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fto is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fto captured in well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Component:
  • Fto Microplate: 96 Wells (12 x 8 Well strips)
  • Fto Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Fto Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.086 ng/mL
• Specificity: Natural and recombinant Rat Alpha-ketoglutarate-dependent dioxygenase FTO
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fto
• Gene Full Name: FTO, alpha-ketoglutarate dependent dioxygenase
• Alias Symbols: alpha-ketoglutarate-dependent dioxygenase FTO;fat mass and obesity associated;fat mass and obesity-associated protein;m6A(m)-demethylase FTO;mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO;mRNA N(6)-methyladenosine demethylase FTO;protein fto;RGD1305121;tRNA N1-methyl adenine demethylase FTO;U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO;U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO.
• NCBI Gene Id: 291905
• Protein Name: Q2A121
• Description of Target: Alpha-ketoglutarate-dependent dioxygenase FTO
• Uniprot ID: https://www.uniprot.org/uniprot/Q2A121
• Protein Accession #: NP_001034802.1
• Nucleotide Accession #: NM_001039713.1
• Protein Size (# AA): 502
• Molecular Weight: 58 kDa