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Catalog No: OKCD09143
Size:96WELLS
Price: $825.00
SKU
OKCD09143
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Datasheets/ManualsPrintable datasheet for FSTL3 ELISA Kit (Mouse) (OKCD09143)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follistatin Like Protein 3 (FSTL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Follistatin Like Protein 3 (FSTL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Follistatin Like Protein 3 (FSTL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Follistatin Like Protein 3 (FSTL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin Like Protein 3 (FSTL3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin Like Protein 3 (FSTL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
FSTL3 Microplate96 Wells (12 x 8 Well strips)
FSTL3 Lyophilized Standard2
100X FSTL3 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 6.4pg/mL
Gene SymbolFstl3
Gene Full Namefollistatin-like 3
Alias SymbolsE030038F23Rik;Fl;Flrg;follistatin-like protein 3;follistatin-related gene protein;follistatin-related protein 3.
NCBI Gene Id83554
Protein NameFollistatin-related protein 3
Description of TargetThe secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiation. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. The nuclear form is probably involved in transcriptional regulation via interaction with MLLT10 (By similarity).
Uniprot IDQ9EQC7
Protein Accession #NP_113557.1
Nucleotide Accession #NM_031380.2
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