Catalog No: OKEH07965
Size:96T
Price: $800.00
SKU
OKEH07965
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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FSTL1 ELISA Kit (Bovine) (OKEH07965)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology FSTL1 ELISA Kit (Bovine) (OKEH07965) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FSTL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FSTL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FSTL1 captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.4% (n = 20)
Mean Inter-assay CV%: <=5.9% (n = 20)
ELISA Kit Component
ComponentAmount
FSTL1 Microplate96 Wells (12 x 8 Well strips)
FSTL1 Lyophilized Standard2
100X Biotinylated FSTL1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
::Subcellular Location: Secreted
::Unigene: Bt.89210
SMR: Q58D84
String: 9913.ENSBTAP00000029886
::KEGG: bta:101909195
Pfam: PF09289
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Bovine Follistatin-related protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFSTL1
Gene Full Namefollistatin like 1
Alias Symbolsfollistatin-like protein 1;follistatin-related protein 1.
NCBI Gene Id534482
Protein NameQ58D84
Description of TargetFollistatin-related protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q58D84
Protein Accession #NP_001017950.1
Nucleotide Accession #NM_001017950.2
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