Catalog No: OKEH00618
Size:96T
Price: $375.00
SKU
OKEH00618
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FSHB ELISA Kit (Pig) (OKEH00618) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FSHB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FSHB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FSHB captured in well.
ELISA Kit Range0.78-50mIU/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.2% (n = 20)
Mean Inter-assay CV%: < 10.1% (n = 20)
ELISA Kit Component
ComponentAmount
FSHB Microplate96 Wells (12 x 8 Well strips)
FSHB Lyophilized Standard2
100X Biotinylated FSHB Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.17mIU/mL
SpecificityNatural and recombinant Pig Follitropin subunit beta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFSHB
Gene Full Namefollicle stimulating hormone subunit beta
Alias Symbolsfollicle stimulating hormone beta subunit;follicle stimulating hormone, beta polypeptide;Follicle-stimulating hormone beta subunit;follitropin beta chain;follitropin beta subunit;follitropin subunit beta;FSH-B;FSH-beta.
NCBI Gene Id396895
Protein NameP01228
Description of TargetFollitropin subunit beta
Uniprot IDhttps://www.uniprot.org/uniprot/P01228
Protein Accession #NP_999040.1
Nucleotide Accession #NM_213875.1
Protein Size (# AA)129
Molecular Weight15 kDa
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