Catalog No: OKCA00327
Size:96 Wells
Price: $667.00
SKU
OKCA00327
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FSH ELISA Kit (Sheep) : 96 Wells (OKCA00327)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivitySheep
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:190%85-94%
Serum (n=4)1:284%80-88%
Serum (n=4)1:494%90-99%
Serum (n=4)1:892%87-96%
ELISA Kit PrincipleAviva Systems Biology FSH ELISA Kit (Sheep) (OKCA00327) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Sheep monoclonal antibody specific for Sheep FSH has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Sheep FSH is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sheep FSH captured in well.
ELISA Kit Range2 mI U/mL - 70mI U/mL
ELISA Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n = 5)0.9792-101%
Sample 2
Sample TypeAverage RecoveryRange
EDTA plasma (n = 4)0.9591-99%
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (mIU/mL)10.0029.55810.3029.30110.7579.119
SD0.0260.0280.0280.0360.0420.036
CV%6.3747.126.8549.3829.8879.562
ELISA Kit Component
ComponentAmount
Anti-FSH Microplate96 Well (12 x 8 Well Strips)
FSH Standard6 x 500 uL
HRP-Conjugate 1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity1 mIU/ml
SpecificityThis assay has high sensitivity and excellent specificity for detection of sheep
FSH. No significant cross-reactivity or interference between sheep FSH and
analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolFSH
Gene Full Namefollicle stimulating hormone, beta polypeptide
NCBI Gene Id443387
Protein NameFollitropin subunit beta
Description of TargetThe pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein.
Uniprot IDP01227
Protein Accession #NP_001009798.1
Nucleotide Accession #NM_001009798.1
Protein Size (# AA)129
Molecular Weight15 kDa
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