FRZB ELISA Kit (Human) (OKCD01885)

Catalog No: OKCD01885 (Formerly GWB-KBBVT8)

Size:96WELLS

Human FRZB ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	88-97%	78-91%	95-102%	94-103%
  EDTA Plasma (n=5)	89-99%	87-101%	95-105%	97-105%
  Heparin Plasma (n=5)	92-101%	86-96%	80-104%	84-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frizzled Related Protein (FRZB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frizzled Related Protein (FRZB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Frizzled Related Protein (FRZB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Frizzled Related Protein (FRZB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	89-97	93
  EDTA Plasma (n=5)	85-97	90
  Heparin Plasma (n=5)	94-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Frizzled Related Protein (FRZB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Frizzled Related Protein (FRZB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FRZB Microplate: 96 Wells (12 x 8 Well Strips)
  • FRZB Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated FRZB Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 13.3 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Frizzled Related Protein (FRZB). No significant cross-reactivity or interference between Frizzled Related Protein (FRZB) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FRZB
• Gene Full Name: frizzled related protein
• Alias Symbols: FRE;frezzled;FRITZ;frizzled homolog-related;frizzled-related protein 1;FRP-3;FRZB1;FRZB-1;FRZB-PEN;FZRB;hFIZ;OS1;secreted frizzled-related protein 3;sFRP-3;SFRP3;SRFP3.
• NCBI Gene Id: 2487
• Protein Name: Secreted frizzled-related protein 3
• Description of Target: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.
• Uniprot ID: Q92765
• Protein Accession #: NP_001454.2
• Nucleotide Accession #: NM_001463.3.
• Protein Size (# AA): 325