Catalog No: OKCA00355
Size:96 Wells
Price: $550.00
SKU
OKCA00355
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityFish
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva Systems Biology Free-Thyroxine 4 ELISA Kit (Fish) (OKCA00355) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Fish Free-Thyroxine 4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Free-Thyroxine 4 and incubated. The Free-Thyroxine 4 found in the sample or standards competes with the biotinylated Free-Thyroxine 4 for limited binding sites on the immobilized anti-Fish Free-Thyroxine 4 antibody. Excess unbound biotinylated Free-Thyroxine 4 and sample or standard Free-Thyroxine 4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Free-Thyroxine 4 captured in the well and inversely proportional to the amount of Free-Thyroxine 4 which was contained in the sample or standard.
ELISA Kit Range4 pmol/l - 64 pmol/l
ELISA Kit Reproducibility
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Sample123123
n202020202020

Mean (pmolL

16.07516.1316.22416.16315.98616.104
SD0.0390.0420.0350.0520.0550.06
CV%4.5614.9284.1086.0086.3236.947
ELISA Kit Component
ComponentAmount
Anti- Free-Thyroxine 4 Microplate96 Well (12 x 8 Well Strips)
Free-Thyroxine 4 Standard 5 x 1 mL
Free-Thyroxine 4-Biotin Complex1 x 6 mL
Avidin-HRP Conjugate1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma
Sensitivity1.08 pmol/L
SpecificityThis assay has high sensitivity and excellent specificity for detection of fish FT4.
No significant cross-reactivity or interference between fish FT4 and analogues
was observed.
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolFree-Thyroxine 4
Alias SymbolsT4, Thyroxine, T4
Protein NameFree-Thyroxine 4
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