FPR1 ELISA Kit (Human) (OKCD08328)

Catalog No: OKCD08328

Size:96WELLS

FPR1 ELISA Kit (Human) (OKCD08328) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Formyl Peptide Receptor 1 (FPR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Formyl Peptide Receptor 1 (FPR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Formyl Peptide Receptor 1 (FPR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Formyl Peptide Receptor 1 (FPR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Formyl Peptide Receptor 1 (FPR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Formyl Peptide Receptor 1 (FPR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FPR1 Microplate: 96 Wells (12 x 8 Well strips)
  • FPR1 Lyophilized Standard: 2
  • 100X FPR1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.061ng/mL

Target Info

• Gene Symbol: FPR1
• Gene Full Name: formyl peptide receptor 1
• Alias Symbols: fMet-Leu-Phe receptor;FMLP;fMLP receptor;FPR;N-formyl peptide receptor;N-formylpeptide chemoattractant receptor.
• NCBI Gene Id: 2357
• Protein Name: fMet-Leu-Phe receptor
• Description of Target: High affinity receptor for N-formyl-methionyl peptides (fMLP), which are powerful neutrophil chemotactic factors (PubMed:2161213, PubMed:2176894, PubMed:10514456, PubMed:15153520). Binding of fMLP to the receptor stimulates intracellular calcium mobilization and superoxide anion release (PubMed:2161213, PubMed:1712023, PubMed:15153520). This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:1712023, PubMed:10514456).
• Uniprot ID: P21462
• Protein Accession #: NP_001180235.1
• Nucleotide Accession #: NM_001193306.1