FPGS ELISA Kit (Human) (OKWB00206)

Catalog No: OKWB00206

Size:96T

Human FPGS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-101%	85-104%	86-102%	97-100%
  EDTA Plasma (n=5)	83-101%	82-92%	84-97%	83-100%
  Heparin Plasma (n=5)	82-100%	86-98%	81-100%	83-99%

• ELISA Kit Principle: Aviva Systems Biology FPGS ELISA Kit (Human) (OKWB00206) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FPGS has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FPGS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FPGS captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	87-104	94
  EDTA Plasma (n=5)	85-103	95
  Heparin Plasma (n=5)	86-103	93

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • FPGS Microplate: 96 Wells (12 x 8 Well strips)
  • FPGS Lyophilized Standard: 2 x 1 ng
  • 100X FPGS Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 9.4 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FPGS
• Gene Full Name: folylpolyglutamate synthase
• Alias Symbols: folylpoly-gamma-glutamate synthetase;folylpolyglutamate synthase, mitochondrial;tetrahydrofolate synthase;tetrahydrofolylpolyglutamate synthase.
• NCBI Gene Id: 2356
• Protein Name: Folylpolyglutamate synthase, mitochondrial
• Description of Target: Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates.
• Uniprot ID: Q05932
• Protein Accession #: NP_001018088
• Nucleotide Accession #: NM_001018078