FOXP3 Antibody (OAEE00392)

Catalog No: OAEE00392

Size:100UG

Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3).

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: 3G3
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Full-length His-tagged recombinant murine FoxP3
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor, which is the master regulator in the development and function of regulatory T cells.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009 Dec;75(12):1040-50.
  Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6659-64.
  Kuhn A, Beissert S, Krammer PH: CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch Dermatol Res. 2009 Jan;301(1):71-81.
  Barnes MJ, Powrie F: Regulatory T cells reinforce intestinal homeostasis. Immunity. 2009 Sep 18;31(3):401-11.
  Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr Opin Immunol. 2009 Dec;21(6):612-8.
  Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009 Oct 29;114(18):3727-35.
  Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem Immunol Allergy. 2008;94:150-7.

Target Info

• Gene Symbol: FOXP3
• Gene Full Name: forkhead box P3
• Alias Symbols: AIID;DIETER;forkhead box protein P3;FOXP3delta7;immune dysregulation, polyendocrinopathy, enteropathy, X-linked;immunodeficiency, polyendocrinopathy, enteropathy, X-linked;IPEX;JM2;PIDX;Scurfin;XPID.
• NCBI Gene Id: 50943
• Protein Name: Forkhead box protein P3
• Description of Target: Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).
• Uniprot ID: Q9BZS1
• Protein Accession #: NP_001107849.1; NP_054728.2
• Nucleotide Accession #: NM_001114377.1; NM_014009.3