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Catalog No: OCOA03779
Size:20UG
Price: $179.00
SKU
OCOA03779
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FOXO3 Protein Lysate (OCOA03779)

Datasheets/ManualsPrintable datasheet for FOXO3 Protein Lysate (OCOA03779)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionFOXO3 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolFOXO3
Gene Full Nameforkhead box O3
Alias SymbolsAF6q21;AF6q21 protein;FKHRL1;FKHRL1P2;forkhead box O3A;forkhead box protein O3;forkhead homolog (rhabdomyosarcoma) like 1;forkhead in rhabdomyosarcoma-like 1;forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1;FOXO2;FOXO3A;FOXO3A-.
NCBI Gene Id2309
Protein NameNM_201559, NP_963853, O43524
Description of TargetForkhead box protein O3, forkhead box protein O3 [Homo sapiens], Homo sapiens forkhead box O3 (FOXO3), transcript variant 2, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/O43524
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_963853
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_201559
Molecular Weight71.1 kDa
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