FOXM1 ELISA Kit (Human) (OKEH08712)

Catalog No: OKEH08712

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology FOXM1 ELISA Kit (Human) (OKEH08712) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FOXM1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FOXM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FOXM1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.4% (n = 20)
  Mean Inter-assay CV%: <=6.6% (n = 20)
• ELISA Kit Component:
  • FOXM1 Microplate: 96 Wells (12 x 8 Well strips)
  • FOXM1 Lyophilized Standard: 2
  • 100X Biotinylated FOXM1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response.
  Subcellular Location: Nucleus
  Unigene: Hs.239
  SMR: Q08050
  String: 9606.ENSP00000342307
  KEGG: hsa:2305
  MIM: 602341
  Pfam: PF00250
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.069ng/mL
• Specificity: Natural and recombinant Human Forkhead box protein M1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FOXM1
• Gene Full Name: forkhead box M1
• Alias Symbols: FKHL16;forkhead box M1-D;forkhead box protein M1;Forkhead, drosophila, homolog-like 16;forkhead-related protein FKHL16;FOXM1A;FOXM1B;FOXM1C;hepatocyte nuclear factor 3 forkhead homolog 11;HFH11;HFH-11;HNF-3;HNF-3/fork-head homolog 11;INS-1;M-phase phosphoprotein 2;MPHOSPH2;MPM-2 reactive phosphoprotein 2;MPP2;MPP-2;PIG29;transcription factor Trident;TRIDENT;winged-helix factor from INS-1 cells.
• NCBI Gene Id: 2305
• Protein Name: Q08050
• Description of Target: Forkhead box protein M1
• Uniprot ID: https://www.uniprot.org/uniprot/Q08050
• Protein Accession #: NP_001230017.1
• Nucleotide Accession #: NM_001243088.1