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Catalog No: OCOA01967
Size:20UG
Price: $179.00
SKU
OCOA01967
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FOXI1 Protein Lysate (OCOA01967)

Datasheets/ManualsPrintable datasheet for FOXI1 Protein Lysate (OCOA01967)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionFOXI1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolFOXI1
Gene Full Nameforkhead box I1
Alias SymbolsFKH10;FKHL10;forkhead box protein I1;forkhead-like 10;forkhead-related activator 6;forkhead-related protein FKHL10;forkhead-related transcription factor 6;FREAC6;FREAC-6;hepatocyte nuclear factor 3 forkhead homolog 3;HFH3;HFH-3;HNF-3/fork-head homolog-3.
NCBI Gene Id2299
Protein NameE0XEN6, NM_012188, NP_036320, Q12951
Description of TargetForkhead box I1, Forkhead box protein I1, forkhead box protein I1 isoform a [Homo sapiens], Homo sapiens forkhead box I1 (FOXI1), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q12951
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_036320
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_012188
Molecular Weight41 kDa
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