FOXD1 Antibody - N-terminal region (OAAB10686)

Catalog No: OAAB10686

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FOXD1 Antibody (N-term) (Cat. #OAAB10686) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXD1 antibody detected the FOXD1 protein (arrow).

FOXD1 Antibody (N-term) (OAAB10686)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent image of B-3 cell stained with FOXD1 Antibody (N-term)(Cat#OAAB10686, Lot #SA110804BE).B-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXD1 primary antibody (1:25, 1 h at 37C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).FOXD1 immunoreactivity is localized to Nucleus significantly.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: Immunofluorescence, Immunohistochemistry-Paraffin, Western blot
• Additional Information: Function: Transcription factor involved in regulation of gene expression in a variety of processes, including formation of positional identity in the developing retina, regionalization of the optic chiasm, morphogenesis of the kidney, and neuralization of ectodermal cells (By similarity). Involved in transcriptional activation of PGF and C3 genes.
  Cellular Location: Nucleus.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This FOXD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FOXD1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Varies by lot. See vial for concentration.
• Application Info: WB: 1:2000
  IF: 1:25
  IHC-P: 1:10~50
  Optimal dilutions should be determined by the end user.
• Reference: Berg, D.T., et al. J. Biol. Chem. 280(15):14943-14947(2005)
  Ernstsson, S., et al. J. Biol. Chem. 271(35):21094-21099(1996)
  Larsson, C., et al. Genomics 30(3):464-469(1995)
  Pierrou, S., et al. EMBO J. 13(20):5002-5012(1994)
• Publications:

  FOXD1 and Gal-3 Form a Positive Regulatory Loop to Regulate Lung Cancer Aggressiveness. Cancers (Basel). 11, (2019). 31795213

• Description:
  View product citations for FOXD1 Antibody - N-terminal region (OAAB10686) on CiteAb

Target Info

• Gene Symbol: FOXD1
• Gene Full Name: forkhead box D1
• Alias Symbols: Brain factor 2;FKHL8;forkhead box protein D1;Forkhead, drosophila, homolog-like 8;forkhead-like 8;forkhead-related activator 4;forkhead-related protein FKHL8;forkhead-related transcription factor 4;FREAC4;FREAC-4;HFH-BF-2.
• NCBI Gene Id: 2297
• Protein Name: NP_004463.1, Q16676, Q61345
• Description of Target: Forkhead box protein D1, forkhead box protein D1 [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/?query=Q16676+OR+Q61345
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004463.1
• Molecular Weight: 46140