FOXC2 Antibody (OAEB02237)

Catalog No: OAEB02237 (Formerly GWB-6DF32F)

Size:100UG

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed with OAEB02237 (0.1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with OAEB02237 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

OAEB02237 (2ug/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC, WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: FOXC2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%
• Peptide Sequence: RHAAPYSYDCTKY
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Approx 50-55kDa band observed in Human Breast Cancer lysates (calculated MW of 53.7kDa according to NP_005242.1). Recommended concentration: 1-3ug/ml.
• Reference: * Hayashi H, Sano H, Seo S, Kume T.; The Foxc2 transcription factor regulates angiogenesis via induction of integrin beta3 expression.; J Biol Chem. 2008 Aug 29;283(35):23791-800.; Pubmed 18579532
  * Hayashi H, Kume T.; Foxc transcription factors directly regulate Dll4 and Hey2 expression by interacting with the VEGF-Notch signaling pathways in endothelial cells.; PLoS One. 2008 Jun 11;3(6):e2401.; Pubmed 18545664
  * Noon A, Hunter RJ, Witte MH, Kriederman B, Bernas M, Rennels M, Percy D, Enerback S, Erickson RP.; Comparative lymphatic, ocular, and metabolic phenotypes of Foxc2 haploinsufficient and aP2-FOXC2 transgenic mice.; Lymphology. 2006 Jun;39(2):84-94.; Pubmed 16910099
  * Dagenais SL, Hartsough RL, Erickson RP, Witte MH, Butler MG, Glover TW.; Foxc2 is expressed in developing lymphatic vessels and other tissues associated with lymphedema-distichiasis syndrome.; Gene Expr Patterns. 2004 Oct;4(6):611-9.; Pubmed 15465483
  * Dagenais SL, Hartsough RL, Erickson RP, Witte MH, Butler MG, Glover TW.; Foxc2 is expressed in developing lymphatic vessels and other tissues associated with lymphedema-distichiasis syndrome.; Gene Expr Patterns. 2004 Oct;4(6):611-9.; Pubmed 15465483
  * Brice G, Mansour S, Bell R, Collin JR, Child AH, Brady AF, Sarfarazi M, Burnand KG, Jeffery S, Mortimer P, Murday VA.; Analysis of the phenotypic abnormalities in lymphoedema-distichiasis syndrome in 74 patients with FOXC2 mutations or linkage to 16q24.; J Med Genet. 2002 Jul;39(7):478-83.; Pubmed 12114478

Target Info

• Gene Symbol: Foxc2|FOXC2
• Gene Full Name: forkhead box C2
• Alias Symbols: BF-3;brain factor 3;Fkh14;FKHL14;forkhead box C2 (MFH-1, mesenchyme forkhead 1);forkhead box protein C2;forkhead homolog 14;forkhead, Drosophila, homolog-like 14;forkhead-related protein FKHL14;Hfhbf3;LD;mesenchyme fork head protein 1;mesenchyme forkhead 1;Mfh1;MFH-1;MFH-1,mesenchyme forkhead 1;transcription factor FKH-14.
• NCBI Gene Id: 14234|2303
• Protein Name: Forkhead box protein C2
• Uniprot ID: Q99958
• Protein Accession #: NP_005242.1
• Molecular Weight: 53.7