FOXA2 Antibody - middle region (OAAB07364)

Catalog No: OAAB07364

Size:400ul

FOXA2 Antibody (Center) (Cat. #OAAB07364) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FOXA2 antibody detected the FOXA2 protein (arrow).

FOXA2 Antibody (Center) (Cat. #OAAB07364)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXA2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent confocal image of A549 cell stained with FOXA2 Antibody (Center)(Cat#OAAB07364).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXA2 primary antibody (1:25, 1 h at 37C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXA2 immunoreactivity is localized to Nucleus significantly.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IF, IHC
• Additional Information: Function: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity) In embryonic development is required for notochord formation Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc Interacts with the cis-acting regulatory regions of these genes Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation
  Cellular Location: Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This FOXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-157 amino acids from the Central region of human FOXA2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that FOXA2 is expressed in MDA-MB231

• Intended Use: FOXA2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: FOXA2
• Alias Symbols: HNF3B, TCF3B, HNF-3-beta
• NCBI Gene Id: 3170
• Protein Name: Hepatocyte nuclear factor 3-beta
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9Y261
• Protein Accession #: NP_068556.2
• Molecular Weight: 48306 Da