FOXA1 Antibody - middle region (Phospho-S286) (OAAB11122)

Catalog No: OAAB11122

Size:400ul

FOXA1 Antibody (Center S286) (Cat. #OAAB11122) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FOXA1 antibody detected the FOXA1 protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
  Function: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity) Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis- acting regulatory regions of these genes. Involved in glucose homeostasis.
  Cellular Location: Nucleus.
  Tissue Location: Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This FOXA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-298 amino acids from the Central region of human FOXA1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.5 mg/ml (actual concentration varies with each lot)
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: FOXA1
• Alias Symbols: HNF3A, TCF3A
• NCBI Gene Id: 3169
• Protein Name: Hepatocyte nuclear factor 3-alpha
• Description of Target: This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
• Uniprot ID: P55317
• Protein Accession #: NP_004487.2
• Molecular Weight: 49 kDa