Catalog No: OCOA18339
Size:20UG
Price: $169.00
SKU
OCOA18339
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FOSB Protein Lysate (OCOA18339)

Datasheets/ManualsPrintable datasheet for FOSB Protein Lysate (OCOA18339)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionFOSB HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolFOSB
Gene Full NameFosB proto-oncogene, AP-1 transcription factor subunit
Alias Symbolsactivator protein 1;AP-1;FBJ murine osteosarcoma viral oncogene homolog B;FosB proto-oncogene, AP-1 trancription factor subunit;G0/G1 switch regulatory protein 3;G0S3;GOS3;GOSB;protein fosB.
NCBI Gene Id2354
Protein NameNM_001114171, NP_001107643, P53539
Description of TargetHomo sapiens FosB proto-oncogene, AP-1 transcription factor subunit (FOSB), transcript variant 2, mRNA, Protein fosB, protein fosB isoform 2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P53539
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001107643
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001114171
Molecular Weight31.3 kDa
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