FOS ELISA Kit (Human) (OKEH04574)

Catalog No: OKEH04574

Size:96T

Human FOS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology FOS ELISA Kit (Human) (OKEH04574) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FOS has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for FOS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FOS captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay Precision - ≤ 5.8%, n = 20
  Mean Inter-Assay Precision - ≤ 8.9%, n = 20
• ELISA Kit Component:
  • Anti-FOS Microplate: 96 Wells (12 x 8 Well Strips)
  • FOS Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • FOS Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.12 ng/mL
• Specificity: Natural and recombinant Human Proto-oncogene c-Fos
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FOS
• Gene Full Name: Fos proto-oncogene, AP-1 transcription factor subunit
• Alias Symbols: activator protein 1;AP-1;cellular oncogene c-fos;Cellular oncogene fos;C-FOS;FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS);FBJ murine osteosarcoma viral oncogene homolog;Fos proto-oncogene, AP-1 trancription factor subunit;G0/G1 switch regulatory protein 7;p55;proto-oncogene c-Fos.
• NCBI Gene Id: 2353
• Protein Name: P01100
• Description of Target: Proto-oncogene c-Fos
• Uniprot ID: https://www.uniprot.org/uniprot/P01100
• Protein Accession #: NP_005243.1
• Nucleotide Accession #: NM_005252.3
• Protein Size (# AA): 380
• Molecular Weight: 41 KDa