FOS ELISA Kit (Human) (OKCD07290)

Catalog No: OKCD07290

Size:96WELLS

FOS ELISA Kit (Human) (OKCD07290) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FOS Microplate: 96 Wells (12 x 8 Well strips)
  • FOS Lyophilized Standard: 2
  • 100X FOS HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.054ng/mL

Target Info

• Gene Symbol: FOS
• Gene Full Name: Fos proto-oncogene, AP-1 transcription factor subunit
• Alias Symbols: activator protein 1;AP-1;cellular oncogene c-fos;Cellular oncogene fos;C-FOS;FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS);FBJ murine osteosarcoma viral oncogene homolog;Fos proto-oncogene, AP-1 trancription factor subunit;G0/G1 switch regulatory protein 7;p55;proto-oncogene c-Fos.
• NCBI Gene Id: 2353
• Protein Name: Proto-oncogene c-Fos
• Description of Target: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
• Uniprot ID: P01100
• Protein Accession #: NP_005243.1
• Nucleotide Accession #: NM_005252.3