FOS Antibody (Phospho-Thr325) : HRP (OABF01171-HRP)

Catalog No: OABF01171-HRP

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IHC-P, IF
• Additional Information: Subcellular location: Cytoplasm, Nucleus
  Modification: Phosphorylation (Thr325)
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325
• Purification: Purified by Protein A.
• Concentration: 0.6-0.7 mg/ml
• Application Info: WB(1:100-1000)
  IHC-P(1:100-500)
  IF(1:50-200)

Target Info

• Gene Symbol: FOS
• Gene Full Name: FBJ murine osteosarcoma viral oncogene homolog
• Alias Symbols: p55, AP-1, C-FOS
• NCBI Gene Id: 2353
• Description of Target: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
• Uniprot ID: P01100