Catalog No: OCOA02336
Size:20UG
Price: $179.00
SKU
OCOA02336
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FOLR1 Protein Lysate (OCOA02336)

Datasheets/ManualsPrintable datasheet for FOLR1 Protein Lysate (OCOA02336)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionFOLR1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolFOLR1
Gene Full Namefolate receptor alpha
Alias Symbolsadult folate-binding protein;FBP;folate binding protein;Folate receptor 1;folate receptor 1 (adult);folate receptor alpha;folate receptor, adult;FOLR;FRalpha;FR-alpha;KB cells FBP;NCFTD;ovarian tumor-associated antigen MOv18.
NCBI Gene Id2348
Protein NameA0A024R5H1, NM_016725, NP_057937, P15328
Description of TargetFolate receptor 1 (Adult), isoform CRA_a, Folate receptor alpha, folate receptor alpha precursor [Homo sapiens], Homo sapiens folate receptor 1 (FOLR1), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P15328
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_057937
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_016725
Molecular Weight29.82 kDa
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