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Catalog No: OKCD08794
Size:96WELLS
Price: $750.00
SKU
OKCD08794
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Datasheets/ManualsPrintable datasheet for FOLR1 ELISA Kit (Human) (OKCD08794)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Folate Receptor 1, Adult (FOLR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Folate Receptor 1, Adult (FOLR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Folate Receptor 1, Adult (FOLR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Folate Receptor 1, Adult (FOLR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
FOLR1 Microplate96 Wells (12 x 8 Well strips)
FOLR1 Lyophilized Standard2
100X FOLR1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 5.8pg/mL
Gene SymbolFOLR1
Gene Full Namefolate receptor alpha
Alias Symbolsadult folate-binding protein;FBP;folate binding protein;Folate receptor 1;folate receptor 1 (adult);folate receptor alpha;folate receptor, adult;FOLR;FRalpha;FR-alpha;KB cells FBP;NCFTD;ovarian tumor-associated antigen MOv18.
NCBI Gene Id2348
Protein NameFolate receptor alpha
Description of TargetBinds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation.
Uniprot IDP15328
Protein Accession #NP_000793.1
Nucleotide Accession #NM_000802.3
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