FOLR1 ELISA Kit (Human) (OKBB00925)

Catalog No: OKBB00925

Size:96 wells

FOLR1 ELISA Kit (Human) (OKBB00925) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human FOLR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FOLR1 has been precoated onto 96-well plates. Standards(E.coli, R25-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FOLR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human FOLR1 amount of sample captured in plate.
• ELISA Kit Range: 46.9 pg/ml - 3,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	103	333	1306	108	312	1388
  Standard deviation	7.41	22.31	88.8	8.53	24.96	119.36
  CV(%)	7.2	6.7	6.8	7.9	8	8.6

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human FOLR1 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human FOLR1 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human FOLR1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	103	108	100	99	102	3.5	3.4%
  Sample 2	333	359	355	339	346	10.8	3.1%
  Sample 3	1306	1386	1411	1506	1402	71.35	5%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	46.9	94	188	375	750	1500	3000
  O.D.	0.095	0.101	0.131	0.203	0.397	0.692	1.288	2.224

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human FOLR1
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: R25-S234
• Description: For quantitative detection of human FOLR1 in cell culture supernatants, serum, plasma(heparin, EDTA), saliva, urine and human milk.

Target Info

• Gene Symbol: FOLR1
• Gene Full Name: folate receptor 1 (adult)
• Alias Symbols: Adult folate binding protein, Adult folate-binding protein, FBP, Folate Receptor 1 Adult, Folate receptor 1, Folate Receptor 1 Precursor, Folate receptor adult, Folate receptor alpha, Folate receptor, FOLR, FOLR1, FOLR1_HUMAN, FR alpha, FR-alpha, FRalpha, KB cells FBP, MOV18, Ovarian cancer associated antigen, Ovarian tumor associated antigen, Ovarian tumor associated antigen MOv18, Ovarian tumor-associated antigen MOv18
• NCBI Gene Id: 2348
• Protein Name: Folate receptor alpha
• Description of Target: Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor(FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein(FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.
• Uniprot ID: P15328
• Protein Accession #: NP_000793.1
• Nucleotide Accession #: NM_000802.3