Follicle-Stimulating Hormone Antibody (Human) ELISA Kit (OKCA00418)

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Technical Documents

Datasheets/Manuals	Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info

Predicted Species Reactivity

Human

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Duration

~ 3.5 Hours

ELISA Kit Principle

Aviva Systems Biology Follicle-Stimulating Hormone Antibody (Human) ELISA Kit (OKCA00418) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Follicle-Stimulating Hormone antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanAntibody is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Follicle-Stimulating Hormone Antibody captured in well.

ELISA Kit Reproducibility

Three different samples of known Follicle-Stimulating Hormone Antibody concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
Intra-Assay CV: <15%
Inter-Assay CV: <15%

ELISA Kit Component

Component	Amount
Follicle-Stimulating Hormone Antibody Microplate	96 Wells (12 x 8 Well strips)
Negative Control	1 x 1 mL
Positive Control	1 x 1 mL
Anti-Human Antibody HRP-Conjugate	1 x 10 mL
Sample Diluent	1 x 20 mL
25X Wash Buffer	1 x 20 mL
Substrate A	1 x 5 mL
Substrate B	1 x 5 mL
Stop Solution	1 x 5 mL