FOLH1 Chemi-Luminescent ELISA Kit (Rat) (OKCD03503)

Catalog No: OKCD03503

Size:96WELLS

Rat FOLH1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	87-96%	87-101%	78-101%	97-105%
  EDTA Plasma (n=5)	89-103%	92-101%	83-95%	82-101%
  Heparin Plasma (n=5)	80-101%	94-101%	92-103%	85-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Granulocyte Chemotactic Protein 2 (GCP2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Granulocyte Chemotactic Protein 2 (GCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Granulocyte Chemotactic Protein 2 (GCP2) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-104	101
  EDTA Plasma (n=5)	86-93	89
  Heparin Plasma (n=5)	94-101	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granulocyte Chemotactic Protein 2 (GCP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granulocyte Chemotactic Protein 2 (GCP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Folh1 Microplate: 96 Wells (12 x 8 Well strips)
  • Folh1 Lyophilized Standard: 2
  • 100X Folh1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Folh1
• Alias Symbols: Naalad
• NCBI Gene Id: 85309
• Protein Name: Glutamate carboxypeptidase 2
• Description of Target: Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides . In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.
• Uniprot ID: P70627
• Protein Accession #: NP_476533
• Nucleotide Accession #: NM_057185