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Datasheets/Manuals | Printable datasheet for FOLH1 Antibody (OAEE00398) (OAEE00398) |
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Predicted Species Reactivity | Human |
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Product Format | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Clonality | Monoclonal |
Clone | GCP-05 |
Isotype | IgG1 |
Host | Mouse |
Application | Flow cytometry|Immunocytochemistry|Immunoprecipitation|Mass cytometry |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | amino acids 44-750 of human GCPII |
Purification | Purified |
Concentration | 1 mg/ml |
Specificity | The antibody GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker. |
Purity | > 95% (by SDS-PAGE) |
Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
Reference | 1. Barinka C, Sacha P, Sklenar J, Man P, Bezouska K, Slusher BS, Konvalinka J: Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity. Protein Sci. 2004, 13: 1627-1635 2. Barinka C, Mlcochova P, Sacha P, Hilgert I, Majer P, Slusher BS, Horejsi V, Konvalinka J: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding. Eur. J. Biochem. 2004, 271: 2782-2790 3. Barinka C, Rinnova M, Sacha P, Rojas C, Majer P, Slusher BS, Konvalinka J: Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II. J. Neurochem. 2002, 80: 477-487 4. Sacha P, Zamecnik J, Barinka C, Hlouchova K, Vicha A, Mlcochova P, Hilgert I, Eckschlager T, Konvalinka J: Expression of glutamate carboxypeptidase II in human brain. Neuroscience 2007, 144: 1361-1372 5. Ghadge GD, Slusher BS, Bodner A, Canto MD, Wozniak K, Thomas AG, Rojas C, Tsukamoto T, Majer P, Miller RJ, Monti AL, Roos RP: Glutamate carboxypeptidase II inhibition protects motor neurons from death in familial amyotrophic lateral sclerosis models. Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9554-9. 6. Mesters JR, Barinka C, Li W, Tsukamoto T, Majer P, Slusher BS, Konvalinka J, Hilgenfeld R: Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer. EMBO J. 2006, 25: 1375-1384 7. Davis MI, Bennett MJ, Thomas LM, Bjorkman PJ: Crystal structure of prostate-specific membrane antigen, a tumor marker and peptidase. Proc. Natl. Acad. Sci. USA 2005, 102: 5981-5986 |
Gene Symbol | FOLH1 |
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Gene Full Name | folate hydrolase 1 |
Alias Symbols | cell growth-inhibiting gene 27 protein;FGCP;Folate hydrolase 1;FOLH;folylpoly-gamma-glutamate carboxypeptidase;GCP2;GCPII;glutamate carboxylase II;glutamate carboxypeptidase 2;glutamate carboxypeptidase II;membrane glutamate carboxypeptidase;mGCP;NAALAD1;NAALADase I;N-acetylated alpha-linked acidic dipeptidase 1;N-acetylated-alpha-linked acidic dipeptidase I;prostate specific membrane antigen variant F;Prostate-specific membrane antigen;PSM;PSMA;pteroylpoly-gamma-glutamate carboxypeptidase. |
NCBI Gene Id | 2346 |
Protein Name | Glutamate carboxypeptidase 2 |
Description of Target | Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression. |
Uniprot ID | Q04609 |
Protein Accession # | NP_001014986.1; NP_001180400.1; NP_001180401.1; NP_001180402.1; NP_004467.1 |
Nucleotide Accession # | NM_001014986.1; NM_001193471.1; NM_001193472.1; NM_001193473.1; NM_004476.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "FOLH1 Antibody (OAEE00398)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "FOLH1 Antibody (OAEE00398)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "FOLH1 Antibody (OAEE00398)" provided in?
This item is provided in "Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "FOLH1 Antibody (OAEE00398)"?
This target may also be called "cell growth-inhibiting gene 27 protein;FGCP;Folate hydrolase 1;FOLH;folylpoly-gamma-glutamate carboxypeptidase;GCP2;GCPII;glutamate carboxylase II;glutamate carboxypeptidase 2;glutamate carboxypeptidase II;membrane glutamate carboxypeptidase;mGCP;NAALAD1;NAALADase I;N-acetylated alpha-linked acidic dipeptidase 1;N-acetylated-alpha-linked acidic dipeptidase I;prostate specific membrane antigen variant F;Prostate-specific membrane antigen;PSM;PSMA;pteroylpoly-gamma-glutamate carboxypeptidase." in publications.
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What is the shipping cost for "FOLH1 Antibody (OAEE00398)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "FOLH1 Antibody (OAEE00398)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "FOLH1 Antibody (OAEE00398)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "FOLH1 Antibody (OAEE00398)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "FOLH1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "FOLH1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "FOLH1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "FOLH1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "FOLH1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "FOLH1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.