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Catalog No: OKCD01981 (Formerly GWB-KBBIU5)
Size:96WELLS
Price: $675.00
SKU
OKCD01981
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Datasheets/ManualsPrintable datasheet for FNTb ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesyltransferase Beta (FNTb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Farnesyltransferase Beta (FNTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesyltransferase Beta (FNTb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Farnesyltransferase Beta (FNTb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Farnesyltransferase Beta (FNTb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Farnesyltransferase Beta (FNTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-FNTB Microplate96 Wells (12 x 8 Well Strips)
FNTB Lyophilized Standard2 x 20 ng
100X Biotinylated FNTB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.122 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Farnesyltransferase Beta (FNTb). No significant cross-reactivity or interference between Farnesyltransferase Beta (FNTb) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolFNTB
Gene Full NameFarnesyltransferase, CAAX box, beta
Alias SymbolsFPTB
NCBI Gene Id2342
Protein NameProtein farnesyltransferase subunit beta
Description of TargetEssential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.
Uniprot IDP49356
Protein Accession #NP_001189487.1
Nucleotide Accession #NM_001202558.1
Protein Size (# AA)437
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