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Catalog No: OKEH01139
Size:96T
Price: $525.00
SKU
OKEH01139
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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FNDC5 ELISA Kit (Rat) : 96 Wells (OKEH01139)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FNDC5 ELISA Kit (Rat) (OKEH01139) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FNDC5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FNDC5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FNDC5 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
FNDC5 Microplate96 Wells (12 x 8 Well strips)
FNDC5 Lyophilized Standard2
100X Biotinylated FNDC5 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity40.9 pg/mL
SpecificityNatural and recombinant Rat Fibronectin type III domain-containing protein 5
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFndc5
Gene Full Namefibronectin type III domain containing 5
Alias Symbolsfibronectin type III domain-containing protein 5;peroxisomal protein.
NCBI Gene Id260327
Protein NameQ8K3V5
Description of TargetFibronectin type III domain-containing protein 5
Uniprot IDhttps://www.uniprot.org/uniprot/Q8K3V5
Protein Accession #NP_001257910.1
Nucleotide Accession #NM_001270981.1
Protein Size (# AA)209
Molecular Weight23 kDa
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