Catalog No: OKCD09394
Size:96WELLS
Price: $750.00
SKU
OKCD09394
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Datasheets/ManualsPrintable datasheet for FNDC5 ELISA Kit (Human) (OKCD09394)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibronectin Type III Domain Containing Protein 5 (FNDC5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibronectin Type III Domain Containing Protein 5 (FNDC5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibronectin Type III Domain Containing Protein 5 (FNDC5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibronectin Type III Domain Containing Protein 5 (FNDC5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibronectin Type III Domain Containing Protein 5 (FNDC5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibronectin Type III Domain Containing Protein 5 (FNDC5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
FNDC5 Microplate96 Wells (12 x 8 Well strips)
FNDC5 Lyophilized Standard2
100X FNDC5 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 6.9pg/mL
Gene SymbolFNDC5
Gene Full Namefibronectin type III domain containing 5
Alias Symbolsfibronectin type III domain-containing protein 5;fibronectin type III repeat-containing protein 2;FRCP2;irisin.
NCBI Gene Id252995
Protein NameFibronectin type III domain-containing protein 5
Description of TargetIrisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.
Uniprot IDQ8NAU1
Protein Accession #NP_001165411.2
Nucleotide Accession #NM_001171940.1
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