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Catalog No: OKCA01668
Size:96 Wells
Price: $700.00
SKU
OKCA01668
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat9286-96
1:29691-101
1:48985-96
1:88883-93
ELISA Kit PrincipleAviva Systems Biology Fn3k ELISA Kit (Mouse) (OKCA01668) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fn3k has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fn3k is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Fn3k captured in the well.
ELISA Kit Range62.5 - 4,000 pg/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)9187-97
EDTA Plasma (n=4)10296-108
ELISA Kit Component
ComponentAmount
Fn3k Microplate96 Wells (12 x 8 Well strips)
Fn3k Lyophilized Standard2 x 4 ng
100X Biotinylated Fn3k Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolFN3K
Gene Full Namefructosamine 3 kinase
Alias Symbols2310074G21Rik
NCBI Gene Id63828
Protein NameFructosamine-3-kinase
Description of TargetMay initiate a process leading to the deglycation of fructoselysine and of glycated proteins. May play a role in the phosphorylation of 1-deoxy-1-morpholinofructose (DMF), fructoselysine, fructoseglycine, fructose and glycated lysozyme.
Uniprot IDQ9ER35
Protein Accession #NP_071297.1
Nucleotide Accession #NM_022014.4
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