FN3K ELISA Kit (Human) (OKCD01046)

Catalog No: OKCD01046 (Formerly GWB-KBBMC1)

Size:96WELLS

Human FN3K ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  EDTA plasma(n=5)	78-92%	99-105%	90-102%	95-102%
  heparin plasma(n=5)	79-91%	97-105%	81-99%	79-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fructosamine-3-Kinase (FN3K). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fructosamine-3-Kinase (FN3K). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fructosamine-3-Kinase (FN3K), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fructosamine-3-Kinase (FN3K) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  EDTA plasma(n=5)	88-99	93
  heparin plasma(n=5)	79-93	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fructosamine-3-Kinase (FN3K) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fructosamine-3-Kinase (FN3K) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FN3K Microplate: 96 Wells (12 x 8 Well Strips)
  • FN3K Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated FN3K Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.114 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fructosamine-3-Kinase (FN3K). No significant cross-reactivity or interference between Fructosamine-3-Kinase (FN3K) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FN3K
• Gene Full Name: fructosamine 3 kinase
• Alias Symbols: fructosamine-3-kinase;protein-psicosamine 3-kinase FN3K;protein-ribulosamine 3-kinase FN3K.
• NCBI Gene Id: 64122
• Protein Name: Fructosamine-3-kinase
• Description of Target: May initiate a process leading to the deglycation of fructoselysine and of glycated proteins. May play a role in the phosphorylation of 1-deoxy-1-morpholinofructose (DMF), fructoselysine, fructoseglycine, fructose and glycated lysozyme.
• Uniprot ID: Q9H479
• Protein Accession #: NP_071441
• Nucleotide Accession #: NM_022158.3
• Protein Size (# AA): 309