Heparin/Platelet Factor 4 Antibody ELISA Kit (Human) (OKCD01299)

Catalog No: OKCD01299

Size:96WELLS

Human Heparin/Platelet Factor 4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h, 30min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-96%	89-102%	78-92%	91-105%
  EDTA Plasma (n=5)	90-97%	89-103%	84-92%	89-99%
  Heparin Plasma (n=5)	97-105%	83-97%	84-104%	91-101%

• ELISA Kit Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Heparin/Platelet Factor 4 Antibody (Anti-HPF4) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Heparin/Platelet Factor 4 Antibody (Anti-HPF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87-101	97
  EDTA Plasma (n=5)	85-102	89
  Heparin Plasma (n=5)	92-104	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Heparin/Platelet Factor 4 Antibody (Anti-HPF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Heparin/Platelet Factor 4 Antibody (Anti-HPF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Heparin/Platelet Factor 4 antibody Microplate: 96 Wells (12 x 8 Well strips)
  • Heparin/Platelet Factor 4 antibody Lyophilized Standard: 2 x XXX
  • 100X Detector Antibody: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.59 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibronectin (FN). No significant cross-reactivity or interference between Fibronectin (FN) and analogues was observed.
• Assay Info: Assay Method: Quantitative Reverse Capture Sandwich ELISA

Target Info

• Gene Symbol: PF4
• Gene Full Name: platelet factor 4
• Alias Symbols: chemokine (C-X-C motif) ligand 4;C-X-C motif chemokine 4;CXCL4;iroplact;oncostatin-A;PF-4;platelet factor 4;SCYB4.
• NCBI Gene Id: 5196
• Protein Name: Platelet factor 4
• Description of Target: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
• Uniprot ID: P02776
• Protein Accession #: NP_001293058.1
• Nucleotide Accession #: NM_002619.3