FN1 ELISA Kit (Horse) (OKCA01064)

Catalog No: OKCA01064

Size:96 Wells

Horse FN1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Horse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  1:1,000	92	88-96
  1:2,000	94	90-98
  1:4,000	102	98-107
  1:8,000	89	84-93

• ELISA Kit Principle: Aviva Systems Biology FN1 ELISA Kit (Horse) (OKCA01064) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FN1 captured in the well.
• ELISA Kit Range: 7.8 - 500 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	98	94-102
  EDTA Plasma (n=4)	85	80-90

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01064 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01064 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • FN1 Microplate: 96 Wells (12 x 8 Well strips)
  • FN1 Lyophilized Standard: 2 x 500 ng
  • 100X Biotinylated FN1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.95 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: FN1
• Gene Full Name: Fibronectin
• Alias Symbols: CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, OTTHUMP00000206762|OTTHUMP00000206767|cold-insoluble globulin|migration-stimulating fa
• Protein Name: Fibronectin
• Description of Target: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.Isoform 2 is probably involved in matrix organization of cartilage.
• Uniprot ID: Q28377
• Protein Accession #: XP_001489154.4
• Nucleotide Accession #: XM_001489104.5