FMOD ELISA Kit (Rat) (OKCD02458)

Catalog No: OKCD02458

Size:96WELLS

Rat FMOD ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-94%	86-102%	87-101%	80-95%
  EDTA Plasma (n=5)	78-93%	98-105%	80-90%	89-103%
  Heparin Plasma (n=5)	95-103%	78-91%	84-105%	79-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibromodulin (FMOD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibromodulin (FMOD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibromodulin (FMOD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibromodulin (FMOD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-103	99
  EDTA Plasma (n=5)	87-103	94
  Heparin Plasma(n=5)	92-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibromodulin (FMOD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibromodulin (FMOD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FMOD Microplate: 96 Wells (12 x 8 Well strips)
  • FMOD Lyophilized Standard: 2
  • 100X Biotinylated FMOD Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibromodulin (FMOD). No significant cross-reactivity or interference between Fibromodulin (FMOD) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fmod
• Gene Full Name: fibromodulin
• Alias Symbols: collagen-binding 59 kDa protein;fibromodulin;FM;keratan sulfate proteoglycan fibromodulin;KSPG fibromodulin.
• NCBI Gene Id: 64507
• Protein Name: Fibromodulin
• Description of Target: Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis (By similarity).
• Uniprot ID: P50609
• Protein Accession #: NP_542429.1
• Nucleotide Accession #: NM_080698.1
• Protein Size (# AA): 376