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Catalog No: OKEH04480
Size:96T
Price: $525.00
SKU
OKEH04480
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FMOD ELISA Kit (Human) (OKEH04480) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FMOD has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for FMOD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FMOD captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.1% (n = 20)
Mean Inter-assay CV%: <= 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-FMOD Microplate96 Wells (12 x 8 Well Strips)
FMOD Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
FMOD Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Human Fibromodulin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFMOD
Gene Full Namefibromodulin
Alias Symbolscollagen-binding 59 kDa protein;epididymis secretory sperm binding protein;fibromodulin;FM;keratan sulfate proteoglycan fibromodulin;KSPG fibromodulin;SLRR2E.
NCBI Gene Id2331
Protein NameQ06828
Description of TargetFibromodulin
Uniprot IDhttps://www.uniprot.org/uniprot/Q06828
Protein Accession #NP_002014.2
Nucleotide Accession #NM_002023.4
Protein Size (# AA)376
Molecular Weight43 KDa
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