Catalog No: OKCD03494
Size:96WELLS
Price: $800.00
SKU
OKCD03494
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FLT3LG Chemi-Luminescent ELISA Kit (Human) (OKCD03494)

Datasheets/ManualsPrintable datasheet for FLT3LG Chemi-Luminescent ELISA Kit (Human) (OKCD03494)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)78-95%78-101%86-102%86-95%
EDTA Plasma (n=5)82-95%89-99%88-102%84-102%
Heparin Plasma (n=5)96-105%80-101%83-93%87-96%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to FMS Like Tyrosine Kinase 3 Ligand (Flt3L). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FMS Like Tyrosine Kinase 3 Ligand (Flt3L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the FMS Like Tyrosine Kinase 3 Ligand (Flt3L) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)96-104101
EDTA Plasma (n=5)89-9793
Heparin Plasma (n=5)82-9994
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 Ligand (Flt3L) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 Ligand (Flt3L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
FLT3LG Microplate96 Wells (12 x 8 Well strips)
FLT3LG Lyophilized Standard2
100X FLT3LG Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.63 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFLT3LG
Gene Full Namefms related receptor tyrosine kinase 3 ligand
Alias SymbolsFL;FLG3L;flt3 ligand;FLT3L;fms related tyrosine kinase 3 ligand;fms-related tyrosine kinase 3 ligand;SL cytokine.
NCBI Gene Id2323
Protein NameFms-related tyrosine kinase 3 ligand
Description of TargetStimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Uniprot IDP49771
Protein Accession #NP_001191431
Nucleotide Accession #NM_001204502
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