Catalog No: OPPA00670 (Formerly GWB-ADD65C)
Size:2UG
Price: $75.00
SKU
OPPA00670
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Flt3 Ligand Protein (OPPA00670)

Datasheets/ManualsPrintable datasheet for OPPA00670
Product Info
Predicted Species ReactivityHuman
Product FormatThe protein was lyophilized with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
HostBaculovirus, SF9 Insect Cells
Additional InformationSolubility: It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
::Biological Activity: The ED50, calculated by the dose-dependant stimulation of the proliferation of human MUTZ-2cells is < 3.0 ng/ml.
::Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.42 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard of Flt3-Ligand as a Reference Standard.
Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.42 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard of Flt3-Ligand as a Reference Standard.
::Product Introduction: FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
Product Description: Flt3-Ligand Human Recombinant produced in Sf9 insect cells is a glycosylated, polypeptide chain containing 155 amino acids and having migrating on SDS-PAGE at 19kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.
Reconstitution and StorageLyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Flt3-L should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
PurityGreater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Peptide SequenceThe sequence of the first five N - terminal amino acids was determined and was found to be Thr - Gln - Asp - Cys - Ser.
SourceSf9 Cells
Gene SymbolFlt3 Ligand Sf9
Alias SymbolsFL, FLG3L, FLT3L
NCBI Gene Id2323
Protein NameFms-related tyrosine kinase 3 ligand
Description of TargetRecombinant Human Flt3-Ligand Sf9
Uniprot IDP49771
Protein Accession #NP_001450.2
Protein Size (# AA)Recombinant
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