Catalog No: OPPA00670 (Formerly GWB-ADD65C)
Size:2UG
Price: $75.00
SKU
OPPA00670
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/Manuals | Printable datasheet for OPPA00670 |
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Predicted Species Reactivity | Human |
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Product Format | The protein was lyophilized with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Host | Baculovirus, SF9 Insect Cells |
Additional Information | Solubility: It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
:: | Biological Activity: The ED50, calculated by the dose-dependant stimulation of the proliferation of human MUTZ-2cells is < 3.0 ng/ml. |
:: | Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.42 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard of Flt3-Ligand as a Reference Standard. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.42 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard of Flt3-Ligand as a Reference Standard. |
:: | Product Introduction: FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation. Product Description: Flt3-Ligand Human Recombinant produced in Sf9 insect cells is a glycosylated, polypeptide chain containing 155 amino acids and having migrating on SDS-PAGE at 19kDa. Flt3-Ligand is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Flt3-L should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Peptide Sequence | The sequence of the first five N - terminal amino acids was determined and was found to be Thr - Gln - Asp - Cys - Ser. |
Source | Sf9 Cells |
Gene Symbol | Flt3 Ligand Sf9 |
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Alias Symbols | FL, FLG3L, FLT3L |
NCBI Gene Id | 2323 |
Protein Name | Fms-related tyrosine kinase 3 ligand |
Description of Target | Recombinant Human Flt3-Ligand Sf9 |
Uniprot ID | P49771 |
Protein Accession # | NP_001450.2 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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