FLT1 ELISA Kit (Pig) (OKCD00849)

Catalog No: OKCD00849 (Formerly GWB-KBBND7)

Size:96WELLS

Pig Flt1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	80-91%	79-89%	88-104%	79-98%
  EDTA plasma(n=5)	99-105%	80-95%	91-103%	93-104%
  heparin plasma(n=5)	98-105%	78-89%	89-103%	83-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor Receptor 1 (VEGFR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor Receptor 1 (VEGFR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor Receptor 1 (VEGFR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	79-98	93
  EDTA plasma(n=5)	93-105	99
  heparin plasma(n=5)	90-97	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Flt1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Flt1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Flt1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.117 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor Receptor 1 (VEGFR1). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FLT1
• Gene Full Name: FMS-related tyrosine kinase 1
• Alias Symbols: FLT-1, VEGFR-1
• NCBI Gene Id: 54251
• Protein Name: vascular endothelial growth factor receptor 1, partial [Sus scrofa]
• Description of Target: vascular endothelial growth factor receptor 1, partial [Sus scrofa]
• Uniprot ID: P53767
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/ACH73486.1
• Nucleotide Accession #: NM_001309381.1
• Protein Size (# AA): 1336