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Datasheets/Manuals | Printable datasheet for FLT1 Antibody (Phospho-Tyr1048) (OAAF00486) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human VEGFR1 around the phosphorylation site of Tyr1048. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | VEGFR1 (Phospho-Tyr1048) antibody detects endogenous levels of VEGFR1 only when phosphorylated at tyrosine 1048. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; ELISA: 1:1000 |
Reference | Shibuya M., Oncogene 5:519-524(1990). Kendall R.L., Proc. Natl. Acad. Sci. U.S.A. 90:10705-10709(1993). The MGC Project Team; Genome Res. 14:2121-2127(2004). |
Gene Symbol | FLT1 |
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Gene Full Name | fms related receptor tyrosine kinase 1 |
Alias Symbols | FLT;FLT-1;fms related tyrosine kinase 1;fms-like tyrosine kinase 1;fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor);tyrosine-protein kinase FRT;tyrosine-protein kinase receptor FLT;vascular endothelial growth factor receptor 1;vascular permeability factor receptor;VEGFR1;VEGFR-1. |
NCBI Gene Id | 2321 |
Protein Name | Vascular endothelial growth factor receptor 1 |
Description of Target | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 7 has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion. |
Uniprot ID | P17948 |
Protein Accession # | NP_001153392.1 |
Molecular Weight | 150 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "FLT1 Antibody (Phospho-Tyr1048) (OAAF00486)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "FLT1 Antibody (Phospho-Tyr1048) (OAAF00486)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "FLT1 Antibody (Phospho-Tyr1048) (OAAF00486)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "FLT1 Antibody (Phospho-Tyr1048) (OAAF00486)"?
This target may also be called "FLT;FLT-1;fms related tyrosine kinase 1;fms-like tyrosine kinase 1;fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor);tyrosine-protein kinase FRT;tyrosine-protein kinase receptor FLT;vascular endothelial growth factor receptor 1;vascular permeability factor receptor;VEGFR1;VEGFR-1." in publications.
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What is the shipping cost for "FLT1 Antibody (Phospho-Tyr1048) (OAAF00486)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "FLT1 Antibody (Phospho-Tyr1048) (OAAF00486)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "FLT1 Antibody (Phospho-Tyr1048) (OAAF00486)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "150 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "FLT1 Antibody (Phospho-Tyr1048) (OAAF00486)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "FLT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "FLT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "FLT1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "FLT1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "FLT1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "FLT1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.