FLRG/FSTL3 ELISA Kit (Human) (OKBB00639)

Catalog No: OKBB00639

Size:96 Wells

Human FLRG/FSTL3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human FLRG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FLRG has been precoated onto 96-well plates. Standards(NS0, M27-V263) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FLRG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human FLRG amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	0.52	1.13	2.28	0.57	1.24	2.36
  SD	0.022	0.044	0.093	0.043	0.084	0.175
  CV%	4.2	3.9	4.1	7.5	6.8	7.4

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human FLRG Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human FLRG Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human FLRG Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA), urine and human milk
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human FLRG
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: M27-V263
• Description: For quantitative detection of human FLRG in cell culture supernatants, serum, plasma(heparin, EDTA), urine and human milk.

Target Info

• Gene Symbol: FSTL3
• Gene Full Name: follistatin-like 3 (secreted glycoprotein)
• Alias Symbols: FLRG, follistatin-like 3, follistatin-like 3 glycoprotein, Follistatin-like protein 3, Follistatin-related gene protein, Follistatin-related protein 3, FSRP, Fstl3, FSTL3_HUMAN
• NCBI Gene Id: 10272
• Protein Name: Follistatin-related protein 3
• Description of Target: FSTL3 (Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins thought to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. The FSTL3 gene was localized to chromosome 19p13. This gene involved in hematopoiesis and in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. It may have a role in leukemogenesis.
• Uniprot ID: O95633
• Protein Accession #: NP_005851.1
• Nucleotide Accession #: NM_005860.2