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Catalog No: OKEH03630
Size:96T
Price: $625.00
SKU
OKEH03630
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FLOT2 ELISA Kit (Human) (OKEH03630) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FLOT2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for FLOT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FLOT2 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.7% (n = 20)
Mean Inter-assay CV%: <= 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
FLOT2 Microplate96 Wells (12 x 8 Well strips)
FLOT2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FLOT2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.417 ng/mL
SpecificityNatural and recombinant Human Flotillin-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFLOT2
Gene Full Nameflotillin 2
Alias SymbolsECS1;ECS-1;epidermal surface antigen;ESA;ESA1;Flotillin 2 (epidermal surface antigen 1);flotillin-2;M17S1;membrane component chromosome 17 surface marker 1;membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1).
NCBI Gene Id2319
Protein NameQ14254
Description of TargetFlotillin-2
Uniprot IDhttps://www.uniprot.org/uniprot/Q14254
Protein Accession #NP_004466.2
Nucleotide Accession #NM_004475.2
Protein Size (# AA)428
Molecular Weight47 kDa
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