Catalog No: OKCD08793
Size:96WELLS
Price: $800.00
SKU
OKCD08793
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Datasheets/ManualsPrintable datasheet for FLOT1 ELISA Kit (Human) (OKCD08793)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flotillin 1 (FLOT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Flotillin 1 (FLOT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Flotillin 1 (FLOT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Flotillin 1 (FLOT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Flotillin 1 (FLOT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Flotillin 1 (FLOT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
FLOT1 Microplate96 Wells (12 x 8 Well strips)
FLOT1 Lyophilized Standard2
100X FLOT1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 1.18ng/mL
Gene SymbolFLOT1
Gene Full Nameflotillin 1
Alias Symbolsflotillin-1;integral membrane component of caveolae.
NCBI Gene Id10211
Protein NameFlotillin-1
Description of TargetMay act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Uniprot IDO75955
Protein Accession #NP_001305804.1
Nucleotide Accession #NM_001318875.1
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